rabbit anti human gapdh ab  (Novus Biologicals)

Journal: Journal of Clinical and Translational Hepatology

Article Title: Establishment of a Prognostic Model for Hepatocellular Carcinoma Based on Bioinformatics and the Role of NR6A1 in the Progression of HCC

doi: 10.14218/JCTH.2022.00191

Figure Lengend Snippet: (A) Transfection of Huh7 cells with an NR6A1-expressing plasmid. GAPDH was used as the internal control. (B) CCK8 assays show that upregulating NR6A1 promotes the proliferation of Huh7 cells. (C), Increased number of cell colonies formed from transfected Huh7 cells, compared with the control cells transfected with an empty vector. (D) Wound healing assays show that upregulation of NR6A1 expression promoted Huh7 cell migra€n. (E) Transwell invasion assay show that upregulation of NR6A1 expression promoted Huh7 cell migration. * p <0.05.

Article Snippet: The primary antibodies were rabbit anti-human anti-GAPDH (1:3,000, Bioss, Beijing, China) and anti-NR6A1 (1:1,000, Proteintech, Illinois, USA) antibodies.

Techniques: Transfection, Expressing, Plasmid Preparation, Transwell Invasion Assay, Migration

Journal: International Journal of Molecular Sciences

Article Title: Anti-Inflammatory Effects of 1,25(OH)2D/Calcitriol in T Cell Immunity: Does Sex Make a Difference?

doi: 10.3390/ijms23169164

Figure Lengend Snippet: Calcitriol upregulates VDR expression in activated peripheral blood T lymphocytes from female and male healthy donors. VDR levels were evaluated by Western blot analysis of T cell lysates after 48 h of calcitriol treatment. ( A ) Blots shown are representative of experiments performed in T cells from 10 randomly selected female and male healthy subjects. ( B ) Densitometry analysis of VDR levels relative to glyceraldehyde 3−phosphate dehydrogenase (GAPDH) is shown. Results are shown as mean ± SD. * p < 0.05 versus untreated cells.

Article Snippet: To ensure the presence of equal amounts of protein, the membranes were re-proved with rabbit anti-human glyceraldehyde 3-phosphate dehydrogenase Ab (GAPDH, Sigma).

Techniques: Expressing, Western Blot

Journal: Stem Cell Research & Therapy

Article Title: Differential expression profiles of long noncoding RNAs and mRNAs in human bone marrow mesenchymal stem cells after exposure to a high dosage of dexamethasone

doi: 10.1186/s13287-020-02040-8

Figure Lengend Snippet: The sequences of primers for qRT-PCR

Article Snippet: The primary antibody for rabbit anti-human GAPDH and all secondary antibodies were purchased from Elabscience (Shanghai, China).

Techniques: Sequencing

Journal: Stem Cell Research & Therapy

Article Title: Differential expression profiles of long noncoding RNAs and mRNAs in human bone marrow mesenchymal stem cells after exposure to a high dosage of dexamethasone

doi: 10.1186/s13287-020-02040-8

Figure Lengend Snippet: Effect of Dex on the osteogenic and adipogenic differentiation of the hBMSCs. a ARS staining of the hBMSCs after treatment with various concentrations of Dex (10 −8 mol/L, 10 −7 mol/L, and 10 −6 mol/L) for 14 days. b The results of the western blotting analysis showing the protein expression levels of the osteogenic markers (BSPII and Runx-2) after treatment for 14 days, and c quantification of the integrated density of the target bands normalized to GAPDH levels. d The results of the qRT-PCR showing the mRNA expression of osteogenic markers (BSPII and Runx-2) after treatment for 14 days, and quantification through normalization to GAPDH levels. e ORO staining of the hBMSCs after treatment with various concentrations of Dex (10 −8 mol/L, 10 −7 mol/L, and 10 −6 mol/L) for 14 days. f The results of the western blotting analysis showing the protein expression levels of the adipogenic markers (PPAR-γ and CEBP-α) after treatment for 14 days, and g quantification of the integrated density of the target bands after normalization to GAPDH levels. h The results of the qRT-PCR showing the mRNA expression levels of adipogenic markers (PPAR-γ and CEBP-α) after treatment for 14 days, and the quantification through normalization to GAPDH levels. Note: all results are presented as mean ± standard deviation of three independent experiments. * P < 0.01 compared with the control group; # P < 0.01 between the two groups. hBMSCs, human bone marrow mesenchymal stem cells; Dex, dexamethasone; BSPII, bone sialoprotein II; Runx-2, runt-related transcription factor-2; PPAR-γ, peroxisome proliferator-activated receptor-γ; CEBP-α, CCAAT/enhancer-binding protein-α

Article Snippet: The primary antibody for rabbit anti-human GAPDH and all secondary antibodies were purchased from Elabscience (Shanghai, China).

Techniques: Staining, Western Blot, Expressing, Quantitative RT-PCR, Standard Deviation, Control, Binding Assay

Journal: Acta Neuropathologica Communications

Article Title: Tenascin-C expression contributes to pediatric brainstem glioma tumor phenotype and represents a novel biomarker of disease

doi: 10.1186/s40478-019-0727-1

Figure Lengend Snippet: Tenascin C (TNC) is Overexpressed in Pediatric Glioma Tissue: TNC expression patterns were evaluated in pediatric glioma tumor and normal brain tissue specimens. a Western blot analysis of protein extract from DIPG tumor tissue and paired normal brain tissue from the same patient demonstrates increased TNC expression in tumor relative to normal specimens. Densitometry analysis of western blot signal intensity confirms statistical significance of TNC overexpression in tumor tissues (* p = 0.0388, paired two-tailed t-test). Data depicted in histogram, with error bars representing standard error of the mean, and in dot plot, with horizontal bars representing mean protein expression levels. Y-axis: Normalized TNC protein expression relative to GAPDH. b Immunohistochemical (IHC) staining of mouse glioma xenograft tumor and normal brain tissue demonstrates TNC staining of the plasma membrane and extracellular matrix in tumor specimens. Scale bar = 50 μm. c IHC staining was strongest in high-grade and midline glioma tissue, compared to low grade glioma and normal brainstem tissue. Scale bar = 100 μm. d H-score semi-quantification of pediatric glioma TNC staining demonstrating correlation with tumor histological grade. Y-axis: TNC H-score from 0 to 300. Horizontal bars represent the mean H-score . e & f Kaplan–Meier estimates of overall survival (e) and progression-free survival (f) stratified by 75% (240) TNC H-score. Higher TNC expression is associated with worse overall and progression-free survival ( p = 0.041 and 0.034, respectively; log-rank test)

Article Snippet: PVDF membranes were blocked using 5% skim milk for one hour, then incubated at 4 °C overnight with rabbit monoclonal anti-human TNC Ab (11,000, Abcam, ab108930) and rabbit monoclonal anti-human GAPDH Ab (15,000, CST, #2118S).

Techniques: Expressing, Western Blot, Over Expression, Two Tailed Test, Immunohistochemical staining, Immunohistochemistry, Staining, Clinical Proteomics, Membrane

Journal: Acta Neuropathologica Communications

Article Title: Tenascin-C expression contributes to pediatric brainstem glioma tumor phenotype and represents a novel biomarker of disease

doi: 10.1186/s40478-019-0727-1

Figure Lengend Snippet: TNC Expression Patterns in Pediatric Glioma Cell Lines. TNC mRNA and protein expression levels were measured in pediatric supratentorial high grade glioma (HGG n = 3) and brainstem glioma (DIPG n = 6) cell lines. a TNC expression determined via quantitative PCR, greatest in DIPGIV (H3.1K27 M) and SF9427 (HGG). b Due to the high level of TNC expressed by SF9427 (H3K27 wild-type), overall TNC mRNA expression was greater in Histone H3K27 wild-type compared to H3K27 M cell lines (** p = 0.0061). a & b Y-axis : Relative TNC mRNA expression level in percent normalized to GAPDH (percent). c TNC protein expression determined via western blot, greatest in DIPGIV and SF7761 (H3.3K27 M mutant lines). d Greater TNC protein expression was seen in Histone H3K27 M compared to wild type cell lines, though this did not reach statistical significance ( p = 0.09). c & d Y-axis : TNC protein expression relative to GAPDH (percent). Error bars represent standard error of the mean

Article Snippet: PVDF membranes were blocked using 5% skim milk for one hour, then incubated at 4 °C overnight with rabbit monoclonal anti-human TNC Ab (11,000, Abcam, ab108930) and rabbit monoclonal anti-human GAPDH Ab (15,000, CST, #2118S).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Mutagenesis